Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells
8.8K Views
•
08:52 min
•
June 13th, 2018
DOI :
June 13th, 2018
•1Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, 2Graduate School of Biomedical Sciences, The University of Texas MD Anderson Cancer Center UTHealth, 3Department of Musculoskeletal Oncology, The First Affiliated Hospital of Sun Yat-sen University, 4Women's Health Institute, Cleveland Clinic Foundation, 5Center for Stem Cell and Regenerative Medicine, The Brown Foundation Institute of Molecular Medicine for the Prevention of Human Diseases, The University of Texas Health Science Center at Houston, 6Center for Precision Health, School of Biomedical Informatics and School of Public Health, The University of Texas Health Science Center at Houston
Chapters in this video
0:04
Title
0:47
Differentiation of Li-Fraumeni Syndrome (LFS) Patient-derived Induced Pluripotent Stem Cell (iPSC) to Mesenchymal Stem Cell (MSC) (Days 0 - 28)
2:19
iPSC-derived MSC Maturation (Day 28 - 45) and Differentiation of LFS MSC to Osteoblasts (Day 0 - 24)
4:33
Xenograft In Vivo Tumorigenesis Model Setup
6:39
Results: Representative LFS MSC Differentiation
8:10
Conclusion
Related Videos
09:25
Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma
18.9K Views
11:15
A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells
10.1K Views
09:01
Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
13.9K Views
08:07
Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
7.1K Views
09:21
Tumorsphere Derivation and Treatment from Primary Tumor Cells Isolated from Mouse Rhabdomyosarcomas
7.1K Views
06:53
Modeling Primary Bone Tumors and Bone Metastasis with Solid Tumor Graft Implantation into Bone
2.6K Views
07:31
A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate
3.5K Views
04:25
Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models
8.7K Views
02:35
Establishment of Patient-Derived Xenograft Mouse Model with Human Osteosarcoma Tissues
646 Views
11:26
Isolation and Immortalization of Patient-derived Cell Lines from Muscle Biopsy for Disease Modeling
16.5K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved