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Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors

DOI :

10.3791/57685-v

June 7th, 2018

June 7th, 2018

9,244 Views

1Department of Otolaryngology-Head and Neck Surgery, Baylor College of Medicine, 2Interdepartmental Graduate Program in Translational Biology and Molecular Medicine, Baylor College of Medicine

Here we describe a method to separate and enrich components of the tumor immune and non-immune microenvironment in established subcutaneous tumors. This technique allows for the separate analysis of tumor immune infiltrate and non-immune tumor fractions which can permit comprehensive characterization of the tumor immune microenvironment.

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Enrichment

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