Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments

4.5K Views

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07:46 min

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April 30th, 2021

DOI :

10.3791/61895-v

April 30th, 2021

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Adele De Ninno¹, Francesca Romana Bertani¹, Annamaria Gerardino¹, Giovanna Schiavoni², Martina Musella³, Claudia Galassi³, Fabrizio Mattei², Antonella Sistigu³^,⁴, Luca Businaro¹

¹CNR Institute for Photonics and Nanotechnology, ²Dept. of Oncology and Molecular Medicine, Istituto Superiore di Sanità, ³Istituto di Patologia Generale, Università Cattolica del Sacro Cuore, ⁴Tumor Immunology and Immunotherapy Unit, IRCCS Regina Elena National Cancer Institute

Summary

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Microfluidic Co culture Models

Immune Response

Tumor Microenvironments

2D Co cultures

3D Co cultures

Tumor immune Cell Crosstalk

Anti cancer Treatments

Real time Visualization

Immune Cell Recruitment

Patient derived Samples

Live Cell Imaging

Incucyte Live Cell Analysis Software

Matrigel

Drug Combination

Fluorescent Dye stained Tumor Cells

Chapters in this video

0:04

Introduction

1:02

2D Chip Cell Plating

2:10

Unlabeled Cancer and Immune Cell Time-Lapse Acquisition

2:43

3D Immuno-Competent Cancer On-Chip Model

5:15

Results I: Migratory and Interaction Patterns of the 2D Tumor-Immune Cells Co-Culture by Time-Lapse Data Analysis

6:19

Results II: Fluorescent Analysis of Infiltrated Immune Cells in a 3D Tumor Microenvironment

6:56

Conclusion

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