Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

10.9K Views

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08:56 min

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July 27th, 2018

DOI :

10.3791/57855-v

July 27th, 2018

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Jeanmarie Verchot¹, Aastha Thapa², Dulanjani Wijayasekara³, Peter R. Hoyt⁴

¹Texas A&M Agrilife Center at Dallas, ²Noble Research Center, Oklahoma State University, ³Department of Biology, College of Engineering and Natural Sciences, The University of Tulsa, ⁴Bioinformatics and Genomics Core Facility, Department of Biochemistry and Molecular Biology, Oklahoma State University

Summary

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Keywords DNA Analysis

Crude Virion Extraction

RNA Analysis

Infected Leaves

New Virus Genomes

Environmental Virology

Badnaviruses

Homogenization

Differential Centrifugation

Chapters in this video

0:04

Title

0:58

General Virus Purification by Differential Centrifugation

4:03

Isolating Virion DNA by Organic Extraction

5:46

Results: Genomes of Two Badnavirus Species Identified from Infected Canna Lilies

7:34

Conclusion

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