Isolation of Extracellular Vesicles from Murine Bronchoalveolar Lavage Fluid Using an Ultrafiltration Centrifugation Technique

9.3K Views

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09:18 min

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November 9th, 2018

DOI :

10.3791/58310-v

November 9th, 2018

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Tanyalak Parimon¹, Norman E. Garrett III¹, Peter Chen¹^,², Travis J. Antes³

¹Department of Medicine, Division of Pulmonary and Critical Care, Women's Guild Lung Institute, Cedars-Sinai Medical Center, ²Department of Biomedical Sciences, Cedars-Sinai Medical Center, ³Department of Medicine, Smidt Heart Institute, Cedars-Sinai Medical Center

Summary

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Keywords Extracellular Vesicles

Bronchoalveolar Lavage Fluid

Ultrafiltration Centrifugation

Ultracentrifugation

Density Gradient Purification

Centrifugal Filter Unit

Retentate

Aliquot

Chapters in this video

0:04

Title

1:15

Murine Bronchoalveolar Lavage Fluid (BALF) Collection and Preparation

2:21

Isolation of Extracellular Vesicles from Murine Bronchoalveolar Lavage Fluid

4:56

Extracellular Vesicle Quantification

5:35

Detection of BALF-derived Extracellular Vesicles

7:16

Results: UFC Method Demonstrates Higher Total Particle Counts and Protein Recover

8:25

Conclusion

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