A Simple Benchtop Filtration Method to Isolate Small Extracellular Vesicles from Human Mesenchymal Stem Cells

1.4K Views

•

09:10 min

•

June 23rd, 2022

DOI :

10.3791/64106-v

June 23rd, 2022

•

Benson Koh¹^,², Kian Leong Tan³, Hong Hao Chan⁴, Qi Hao Daniel Looi², Moon Nian Lim⁵, Chee Wun How⁴, Jia Xian Law¹, Jhi Biau Foo⁶^,⁷

¹Centre for Tissue Engineering and Regenerative Medicine, Universiti Kebangsaan Malaysia Medical Centre, ²My CytoHealth Sdn. Bhd., ³School of Biosciences, Faculty of Health and Medical Sciences, Taylor's University, ⁴School of Pharmacy, Monash University Malaysia, ⁵Haematology Unit, Cancer Research Centre, Institute for Medical Research (IMR), National Institutes of Health (NIH), Ministry of Health Malaysia, ⁶School of Pharmacy, Faculty of Health & Medical Sciences, Taylor's University, ⁷Centre for Drug Discovery and Molecular Pharmacology (CDDMP), Faculty of Health and Medical Sciences, Taylor's University

Summary

Explore More Videos

Benchtop Filtration

Extracellular Vesicles

Mesenchymal Stem Cells

Isolation Protocol

Phosphate buffered Saline

Nanoparticle Tracking Analysis

Cell Debris Removal

Filtration Technique

Characterization

Human Umbilical Cord derived MSCs

Chapters in this video

0:05

Introduction

1:09

Small Extracellular Vesicle Isolation from hUC‐MSCs

2:48

Characterization of hUC‐MSC‐sEVs

7:14

Results: Nanoparticle Tracking Analysis, Biomarker Expression, and Morphology of Single hUC‐MSC‐sEVs

8:39

Conclusion

Related Videos

article

Chromatin Immunoprecipitation from Human Embryonic Stem Cells

20.6K Views

article

Small Volume (1-3L) Filtration of Coastal Seawater Samples

11.1K Views

article

Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells (MSCs) and Endothelial Colony Forming Progenitor Cells (ECFCs)

15.4K Views

article

Implantation of Ferumoxides Labeled Human Mesenchymal Stem Cells in Cartilage Defects

10.5K Views

article

Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

19.4K Views

article

Induction and Analysis of Epithelial to Mesenchymal Transition

35.6K Views

article

Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor

15.1K Views

article

Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury

8.8K Views

article

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

18.0K Views

article

Magnetic-Activated Cell Sorting Strategies to Isolate and Purify Synovial Fluid-Derived Mesenchymal Stem Cells from a Rabbit Model

11.1K Views

Copyright © 2025 MyJoVE Corporation. All rights reserved