RAW 264.7 Cell Phorbol 12-Myristate 13-Acetate (PMA)-Treatment
2:27
Superoxide Detection in Buffer or Cell Samples
3:58
EPR Measurements on Flash Frozen Lung Tissue
4:57
Results: Representative Superoxide Detection
6:21
Conclusion
Transcrição
Dysregulated redox signaling is implicated in the pathogenesis of diverse diseases. The study of redox biology requires the accurate detection of reactive oxygen species in different cellular and tissue compartments. EPR spectroscopy is the only m
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Electron paramagnetic resonance (EPR) spectroscopy is an unambiguous method to measure free radicals. The use of selective spin probes allows for detection of free radicals in different cellular compartments. We present a practical, efficient method to collect biological samples that facilitate treating, storing, and transferring samples for EPR measurements.