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A Syngeneic Mouse B-Cell Lymphoma Model for Pre-Clinical Evaluation of CD19 CAR T Cells

14.5K Views

12:16 min

October 16th, 2018

DOI :

10.3791/58492-v

October 16th, 2018


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Keywords CD19 CAR T Cells

Chapters in this video

0:04

Title

0:55

Days 1 & 2: Preparation and Transfection of Plat-E Retroviral Packaging Cell Line

2:11

Day 3: Preparation of Virus-Containing Supernatant for Transduction and Isolation and In Vitro Activation of Mouse Splenic T Cells

4:27

Day 4: Transduction of Mouse T Cells

6:43

Day 5: Round 2 of Transduction

8:33

In Vitro Validation of CAR T Cell Activity

9:53

Results: Pre-Clinical Evaluation of CD19 CAR T Cells

11:32

Conclusion

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