JoVE Logo
Faculty Resource Center

Sign In

A Syngeneic Mouse B-Cell Lymphoma Model for Pre-Clinical Evaluation of CD19 CAR T Cells

DOI :

10.3791/58492-v

12:16 min

October 16th, 2018

October 16th, 2018

13,794 Views

1Manchester Cancer Research Centre Building, Department Cancer Sciences, University of Manchester, 2Celyad

Here, we present a protocol for the production and pre-clinical testing of murine CD19 CAR T cells by retroviral transduction and utilization as a therapy against established syngeneic A20 B-cell lymphoma in BALB/c mice with or without lymphodepleting pre-conditioning.

Tags

Syngeneic Mouse B cell Lymphoma Model

-- Views

Related Videos

article

A Syngeneic Mouse Model of Metastatic Renal Cell Carcinoma for Quantitative and Longitudinal Assessment of Preclinical Therapies

article

Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma

article

A Syngeneic Pancreatic Cancer Mouse Model to Study the Effects of Irreversible Electroporation

article

Orthotopic Transplantation of Syngeneic Lung Adenocarcinoma Cells to Study PD-L1 Expression

article

A Spheroid Killing Assay by CAR T Cells

article

Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells

article

Manufacturing Chimeric Antigen Receptor (CAR) T Cells for Adoptive Immunotherapy

article

Ultrasound-Guided Orthotopic Implantation of Murine Pancreatic Ductal Adenocarcinoma

article

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate

article

Natural Killer (NK) and CAR-NK Cell Expansion Method using Membrane Bound-IL-21-Modified B Cell Line

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved