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A Syngeneic Mouse B-Cell Lymphoma Model for Pre-Clinical Evaluation of CD19 CAR T Cells

DOI :

10.3791/58492-v

12:16 min

October 16th, 2018

October 16th, 2018

13,532 Views

1Manchester Cancer Research Centre Building, Department Cancer Sciences, University of Manchester, 2Celyad

Here, we present a protocol for the production and pre-clinical testing of murine CD19 CAR T cells by retroviral transduction and utilization as a therapy against established syngeneic A20 B-cell lymphoma in BALB/c mice with or without lymphodepleting pre-conditioning.

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Syngeneic Mouse B cell Lymphoma Model

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