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A Micro-agar Salt Bridge Electrode for Analyzing the Proton Turnover Rate of Recombinant Membrane Proteins

DOI :

10.3791/58552-v

8:09 min

January 7th, 2019

January 7th, 2019

8,338 Views

1Department of Physiology and Biophysics, University of Veterinary Medicine

In electrophysiological measurements, the presence of a diffusion potential disturbs the precise measurement of the reverse potential by altering the electrode potential. Using a micro-agar salt bridge, the impact of the diffusion potential is minimized, which allows a more precise measurement of substrate turnover numbers of reconstituted recombinant membrane proteins.

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Micro agar Salt Bridge Electrode

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