October 11th, 2019
•Our overall aim is to understand how cells sense extracellular cues that lead to directed axonal growth. Here, we describe the methodology of Light-Induced Molecular Adsorption of Proteins, used to produce defined micro-patterns of extracellular matrix components in order to study specific events that govern axon outgrowth and pathfinding.
Tags
Related Videos
Using a Comparative Species Approach to Investigate the Neurobiology of Paternal Responses
Post-embedding Immunogold Labeling of Synaptic Proteins in Hippocampal Slice Cultures
A Caenorhabditis elegans Model System for Amylopathy Study
Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons
Isolation of CA1 Nuclear Enriched Fractions from Hippocampal Slices to Study Activity-dependent Nuclear Import of Synapto-nuclear Messenger Proteins
The Olfactory System as a Model to Study Axonal Growth Patterns and Morphology In Vivo
Using Linear Agarose Channels to Study Drosophila Larval Crawling Behavior
In Utero Electroporation Approaches to Study the Excitability of Neuronal Subpopulations and Single-cell Connectivity
Time-dependent Increase in the Network Response to the Stimulation of Neuronal Cell Cultures on Micro-electrode Arrays
In Vivo Microdialysis Method to Collect Large Extracellular Proteins from Brain Interstitial Fluid with High-molecular Weight Cut-off Probes
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved