In Vitro Method to Control Concentrations of Halogenated Gases in Cultured Alveolar Epithelial Cells

6.5K Views

•

04:56 min

•

October 23rd, 2018

DOI :

10.3791/58554-v

October 23rd, 2018

•

Raïko Blondonnet¹^,², Bertille Paquette¹^,², Damien Richard³, Rémi Bourg²^,⁴, Géraldine Laplace²^,⁴, Romain Segurel²^,⁴, Henria Pouvelle²^,⁴, Corinne Belville², Loic Blanchon², Thomas Godet¹^,², Jean-Michel Constantin¹^,², Jean-Etienne Bazin¹^,², Vincent Sapin², Matthieu Jabaudon¹^,²

¹Department of Perioperative Medicine, CHU Clermont-Ferrand, ²Centre National de la Recherche Scientifique Unité Mixte de Recherche (CNRS UMR) 6293, Institut National de la Santé et de la Recherche Médicale (INSERM) U1103, Laboratoire de Génétique, Reproduction et Développement (GReD), Université Clermont Auvergne, ³Department of Pharmacology, CHU Clermont-Ferrand, ⁴Nurse Anesthetist School, CHU Clermont-Ferrand

Summary

Explore More Videos

Keywords In Vitro

Halogenated Gases

Alveolar Epithelial Cells

Acute Respiratory Distress Syndrome

Airtight Chamber

Chapters in this video

0:04

Title

0:40

Preparation of an Air-tight Chamber

1:57

Expose Alveolar Epithelial Cells to Halogenated Agents

3:13

Results: Concentration of Sevoflurane and Isoflurane Over Time

3:56

Conclusion

Related Videos

article

A Simple Method of Mouse Lung Intubation

50.5K Views

article

Cytotoxic Efficacy of Photodynamic Therapy in Osteosarcoma Cells In Vitro

12.8K Views

article

In vitro Method to Observe E-selectin-mediated Interactions Between Prostate Circulating Tumor Cells Derived From Patients and Human Endothelial Cells

9.0K Views

article

Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies

19.5K Views

article

Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells

12.6K Views

article

Studying the Role of Alveolar Macrophages in Breast Cancer Metastasis

9.6K Views

article

Forskolin-induced Swelling in Intestinal Organoids: An In Vitro Assay for Assessing Drug Response in Cystic Fibrosis Patients

19.1K Views

article

A Method for Quantifying Upper Limb Performance in Daily Life Using Accelerometers

12.3K Views

article

Microscopy Based Methods for the Assessment of Epithelial Cell Migration During In Vitro Wound Healing

9.2K Views

article

Corneal Epithelial Abrasion with Ocular Burr As a Model for Cornea Wound Healing

20.2K Views

Copyright © 2025 MyJoVE Corporation. All rights reserved