Establishment of the Dual Humanized TK-NOG Mouse Model for HIV-associated Liver Pathogenesis
September 11th, 2019
•This protocol provides a reliable method to establish humanized mice with both human immune system and liver cells. Dual reconstituted immunodeficient mice achieved via intrasplenic injection of human hepatocytes and CD34+ hematopoietic stem cells are susceptible to human immunodeficiency virus-1 infection and recapitulate liver damage as observed in HIV-infected patients.
Related Videos
In vitro Biofilm Formation in an 8-well Chamber Slide
Using the BLT Humanized Mouse as a Stem Cell based Gene Therapy Tumor Model
The Insect Galleria mellonella as a Powerful Infection Model to Investigate Bacterial Pathogenesis
Isolation, Purification and Labeling of Mouse Bone Marrow Neutrophils for Functional Studies and Adoptive Transfer Experiments
Establishment of an In vitro System to Study Intracellular Behavior of Candida glabrata in Human THP-1 Macrophages
Humanized Mouse Model to Study Bacterial Infections Targeting the Microvasculature
Two-photon Intravital Imaging of Leukocytes During the Immune Response in Lipopolysaccharide-treated Mouse Liver
Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies
An Ex Vivo Chicken Primary Bursal-cell Culture Model to Study Infectious Bursal Disease Virus Pathogenesis
Evaluating Virulence and Pathogenesis of Aeromonas Infection in a Caenorhabditis elegans Model
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved