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Establishment of the Dual Humanized TK-NOG Mouse Model for HIV-associated Liver Pathogenesis

DOI :

10.3791/58645-v

September 11th, 2019

September 11th, 2019

8,253 Views

1Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, 2Department of Pathology and Microbiology, University of Nebraska Medical Center

This protocol provides a reliable method to establish humanized mice with both human immune system and liver cells. Dual reconstituted immunodeficient mice achieved via intrasplenic injection of human hepatocytes and CD34+ hematopoietic stem cells are susceptible to human immunodeficiency virus-1 infection and recapitulate liver damage as observed in HIV-infected patients.

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