Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing
4.0K Views
•
10:44 min
•
March 23rd, 2022
DOI :
March 23rd, 2022
•Chapters in this video
0:05
Introduction
0:59
Dissection of Adult Mouse Superior Cervical Ganglia
2:12
Dissection of Adult Mouse Stellate Ganglia
2:57
Isolation and Cryopreservation of Mouse Ganglionic Cells
4:56
Nucleus Isolation
6:50
Nucleus Barcoding with Hashtag Oligos and Multiplexing
8:24
Results: Quality Control Analysis of Single-Nucleus cDNA Library Preparation and snRNA-Sequencing
9:55
Conclusion
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