JoVE Logo

Sign In

Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing

3.9K Views

10:44 min

March 23rd, 2022

DOI :

10.3791/63397-v

March 23rd, 2022


Explore More Videos

Nucleus Isolation

Chapters in this video

0:05

Introduction

0:59

Dissection of Adult Mouse Superior Cervical Ganglia

2:12

Dissection of Adult Mouse Stellate Ganglia

2:57

Isolation and Cryopreservation of Mouse Ganglionic Cells

4:56

Nucleus Isolation

6:50

Nucleus Barcoding with Hashtag Oligos and Multiplexing

8:24

Results: Quality Control Analysis of Single-Nucleus cDNA Library Preparation and snRNA-Sequencing

9:55

Conclusion

Related Videos

article

06:22

Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq

12.0K Views

article

07:03

Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications

9.7K Views

article

10:03

Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution

2.2K Views

article

06:38

Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing

18.6K Views

article

09:49

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

10.7K Views

article

08:16

Fluorescence-Activated Nuclei Negative Sorting of Neurons Combined with Single Nuclei RNA Sequencing to Study the Hippocampal Neurogenic Niche

2.7K Views

article

07:00

Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method

24.3K Views

article

06:00

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing

4.3K Views

article

09:09

Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics

5.4K Views

article

07:12

A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing

3.8K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved