Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma

19.6K Views

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07:52 min

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January 9th, 2019

DOI :

10.3791/58711-v

January 9th, 2019

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Guo Hong*¹^,², Shuangyi Fan*³, The Phyu³, Priyanka Maheshwari², Michal Marek Hoppe², Hoang Mai Phuong², Sanjay de Mel⁴, Michelle Poon⁴, Siok-Bian Ng²^,³, Anand D. Jeyasekharan²^,⁴

¹Department of Laboratory Medicine, AnSteel Group General Hospital, ²Cancer Science Institute of Singapore, National University of Singapore, ³Department of Pathology, Yong Loo Lin School of Medicine, National University of Singapore, ⁴Department of Haematology-Oncology, National University Health System

Summary

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Multiplexed Fluorescent Immunohistochemistry

Lymphoma

Antibody based Staining

Tumor Microenvironment

Co expression Assessment

Peroxidase Activity Blocking

Tyramide based Fluorescent Reagent

Primary Antibody Incubation

Secondary Antibody

TBS D Buffer

Chapters in this video

0:04

Title

0:57

Monoplex Tyramide-Based Immunofluorescence

3:22

Monoplex Slide and Tissue Microarray (TMA) Scanning

4:36

Data Analysis

6:28

Results: Representative Mutiplexed Fluorescent Immunohistochemical (mf-IHC) Staining, Imaging, and Analysis

7:10

Conclusion

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