JoVE Logo

Sign In

In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function

11.5K Views

08:04 min

February 27th, 2019

DOI :

10.3791/59275-v

February 27th, 2019


Explore More Videos

In Vitro Tumor Cell Rechallenge

Chapters in this video

0:04

Title

0:54

Tumor Cell: T Cell Co-culture Setup and Tumor Cell Rechallenge

2:37

Sample Harvest for Flow Cytometric Analysis

4:12

Functional and Phenotypic Chimeric Antigen Receptor (CAR) T Cell Analysis

5:14

Results: Representative CD4+ and CD8+ CAR T Cell Phenotypes After Repetitive Challenge

7:30

Conclusion

Related Videos

article

06:08

Assessment of Chimeric Antigen Receptor T Cell-Associated Toxicities Using an Acute Lymphoblastic Leukemia Patient-Derived Xenograft Mouse Model

1.2K Views

article

09:20

Rapid In Vitro Cytotoxicity Evaluation of Jurkat Expressing Chimeric Antigen Receptor using Fluorescent Imaging

2.1K Views

article

07:37

Performing an In Vitro Genome-Wide CRISPR Knockout Screen in Chimeric Antigen Receptor T Cells

721 Views

article

09:56

A Nonviral Approach to Generate Transient Chimeric Antigen Receptor T Cells Using mRNA for Cancer Immunotherapy

377 Views

article

08:46

A Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells

53.0K Views

article

08:09

Droplet-based Cytotoxicity Assay to Assess Chimeric Antigen Receptor T cells at the Single-cell Level

521 Views

article

01:22

An Ex Vivo Technique to Generate Tumor-Specific Chimeric Antigen Receptor T Cells

68 Views

article

01:25

An In Vitro Assay to Assess CAR T Cell Cytotoxicity against Tumor Spheroids

89 Views

article

01:23

An In Vitro Assay to Evaluate the Anti-Tumor Activity of Tumor-Specific CAR T Cells

81 Views

article

01:58

Repetitive Tumor Challenge Assay: An In Vitro Culture Assay to Evaluate Chimeric Antigen Receptor (CAR) T Cells for Repetitive Tumor Killing Potential

1.5K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved