JoVE Logo
Faculty Resource Center

Sign In

Wide-field Single-photon Optical Recording in Brain Slices Using Voltage-sensitive Dye

DOI :

10.3791/59692-v

June 20th, 2019

June 20th, 2019

7,372 Views

1Laboratory for Neural Circuit Systems, Institute of Neuroscience, Tokushima Bunri University

We introduce a reproducible and stable optical recording method for brain slices using voltage-sensitive dye. The article describes voltage-sensitive dye staining and recording of optical signals using conventional hippocampal slice preparations.

Tags

Voltage sensitive Dye

-- Views

Related Videos

article

Optical Imaging of Neurons in the Crab Stomatogastric Ganglion with Voltage-sensitive Dyes

article

Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices

article

Paired Whole Cell Recordings in Organotypic Hippocampal Slices

article

Regioselective Biolistic Targeting in Organotypic Brain Slices Using a Modified Gene Gun

article

Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings

article

A Large Lateral Craniotomy Procedure for Mesoscale Wide-field Optical Imaging of Brain Activity

article

Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method

article

Whole-cell Patch-clamp Recordings in Brain Slices

article

Recording EEG in Freely Moving Neonatal Rats Using a Novel Method

article

Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved