Isolation of Lamina Propria Mononuclear Cells from Murine Colon Using Collagenase E

15.5K Views

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09:48 min

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September 26th, 2019

DOI :

10.3791/59821-v

September 26th, 2019

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Duneia McManus¹^,²^,³^,⁴, Horacio J. Novaira¹^,²^,³^,⁴, Anouk A.J. Hamers¹^,²^,³^,⁴, Asha B. Pillai¹^,²^,³^,⁴^,⁵

¹Department of Pediatrics, Division of Hematology / Oncology and Bone Marrow Transplantation, University of Miami Miller School of Medicine, ²Batchelor Children's Research Institute, University of Miami Miller School of Medicine, ³Department of Microbiology & Immunology, University of Miami Miller School of Medicine, ⁴Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, ⁵Holtz Children's Hospital, University of Miami Miller School of Medicine

Summary

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Lamina Propria

Mononuclear Cells

Murine Colon

Collagenase EIsolation

Immune Cells

Gastrointestinal Disorders

Immune Phenotyping

Intestinal Immune Populations

Protocol Optimization

Colon Dissection

Chilled Colon Buffer

Chapters in this video

0:04

Title

1:00

Harvesting the Colon

1:32

Cleaning the Colon

3:10

Collagenase Digestion 1

3:34

Silica-Based Density Separation Media Gradient Separation

5:44

Collect Mononuclear Cells from the Gradient Interface

6:39

Results: Isolation of Lamina Propria Mononuclear Cells

8:40

Conclusion

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