Preparation and Utilization of Freshly Isolated Human Detrusor Smooth Muscle Cells for Characterization of 9-Phenanthrol-Sensitive Cation Currents

7.1K Views

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08:30 min

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January 31st, 2020

DOI :

10.3791/59884-v

January 31st, 2020

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John Malysz¹, Eric S. Rovner², Robert Wake³, Georgi V. Petkov¹^,³^,⁴

¹Department of Pharmaceutical Sciences, College of Pharmacy, University of Tennessee Health Science Center, ²Department of Urology, Medical University of South Carolina, ³Department of Urology, College of Medicine, University of Tennessee Health Science Center, ⁴Department of Pharmacology, College of Medicine, University of Tennessee Health Science Center

Summary

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Detrusor Smooth Muscle Cells

Urinary Bladder

Cell Isolation Protocol

Electrophysiological Examination

TRPM4 Channel

Nonselective Cation Channel

Patch clamp Electrophysiology

Human Bladder Histology

Cellular Dissection Techniques

Single Cell Analysis

Chapters in this video

0:04

Introduction

1:45

Dissection and Enzymatic Dissociation of Mucosa-free DSM Pieces

3:28

Voltage-step Induced Cation Currents from DSM Cells Using Amphotericin-B Perforated Whole-cell Voltage Patch-clamp Technique

5:52

Results: Investigation of Ion Channel Properties in Isolated DSM Cells

7:07

Conclusion

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