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Ex Vivo Analysis of Mechanically Activated Ca2+ Transients in Urothelial Cells

DOI :

10.3791/64532-v

September 28th, 2022

September 28th, 2022

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1Renal-Electrolyte Division, Department of Medicine, University of Pittsburgh, 2Department of Cell Biology, University of Pittsburgh

This protocol describes a methodology to assess the function of mechanically activated ion channels in native urothelial cells using the fluorescent Ca2+ sensor GCaMP5G.

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