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An Antibody Feeding Approach to Study Glutamate Receptor Trafficking in Dissociated Primary Hippocampal Cultures

5.6K Views

08:13 min

August 2nd, 2019

DOI :

10.3791/59982-v

August 2nd, 2019


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Antibody Feeding Approach

Chapters in this video

0:04

Title

1:13

Live Labeling of Surface-expressed Receptors

1:58

Surface Expression

2:13

Internalization

2:38

Recycling

3:17

Fixation and Labeling of Fixed Cells

5:14

Mounting and Imaging of Samples

5:36

Results: Glutamate Receptor Trafficking in Dissociated Primary Hippocampal Cultures

7:36

Conclusion

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