Bone Marrow Transplantation Platform to Investigate the Role of Dendritic Cells in Graft-versus-Host Disease
7.7K Views
•
08:05 min
•
March 17th, 2020
DOI :
March 17th, 2020
•Hung D. Nguyen*1, Phung Thanh Huong*2, Krystal Hossack1, Sanjeev Gurshaney1, Kevin Ezhakunnel1, Thien-Huong Huynh1, Anamaria Morales Alvarez1, Nhat-Tu Le3, Hung N. Luu4,5
1Cancer Division, Burnett School of Biomedical Sciences, University of Central Florida, 2Department of Biochemistry, Hanoi University of Pharmacy, 3Center for Cardiovascular Regeneration, Department of Cardiovascular Sciences, Houston Methodist Research Institute, 4Department of Epidemiology, University of Pittsburgh Graduate School of Public Health, 5Division of Cancer Control and Population Sciences, University of Pittsburgh Medical Center, Hillman Cancer Center
Chapters in this video
0:04
Introduction
0:49
Graft-Versus-Host Disease (GVHD) Induction
2:53
Donor T Cell Preparation
4:54
Bone Marrow-Derived Dendritic Cell (BM-DC) Preparation
5:58
Results: Representative Effects of Dendritic Cell Function in GVHD
7:26
Conclusion
Related Videos
11:55
Induction of Alloantigen-specific Anergy in Human Peripheral Blood Mononuclear Cells by Alloantigen Stimulation with Co-stimulatory Signal Blockade
14.5K Views
10:29
Induction of Graft-versus-host Disease and In Vivo T Cell Monitoring Using an MHC-matched Murine Model
15.6K Views
08:17
A Human Peripheral Blood Mononuclear Cell (PBMC) Engrafted Humanized Xenograft Model for Translational Immuno-oncology (I-O) Research
14.2K Views
14:43
Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation
17.5K Views
18:48
In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients
14.1K Views
09:50
Induction of Intestinal Graft-versus-host Disease and Its Mini-endoscopic Assessment in Live Mice
9.5K Views
06:06
Induction and Scoring of Graft-Versus-Host Disease in a Xenogeneic Murine Model and Quantification of Human T Cells in Mouse Tissues using Digital PCR
7.0K Views
08:00
Bone Marrow Transplantation Procedures in Mice to Study Clonal Hematopoiesis
12.2K Views
04:29
Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice
8.4K Views
02:57
Bone Marrow-Derived Dendritic Cells Generation: A Method to Generate Dendritic Cells from Mouse Bone Marrow
4.1K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved