Results: Geometry of Human Embryonic Stem Cell Colonies
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Conclusion
Transcript
This protocol allows researchers to culture stem cell colonies on hydrogels with complete control over both the stiffness of the hydrogel and the geometry of the colonies. The main advantage of this technique is that it allows researchers to cultu
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Extracellular matrix ligands can be patterned onto polyacrylamide hydrogels to enable the culture of human embryonic stem cells in confined colonies on compliant substrates. This method can be combined with traction force microscopy and biochemical assays to examine the interplay between tissue geometry, cell-generated forces, and fate specification.