Isolation and Culture of Oculomotor, Trochlear, and Spinal Motor Neurons from Prenatal Isl^mn:GFP Transgenic Mice

10.6K Views

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10:04 min

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November 12th, 2019

DOI :

10.3791/60440-v

November 12th, 2019

•

10,633 Views

Ryosuke Fujiki¹^,²^,³^,⁴^,⁹, Joun Y. Lee¹^,²^,¹⁰, Julie A. Jurgens¹^,²^,³^,⁷, Mary C. Whitman²^,⁵^,⁶, Elizabeth C. Engle¹^,²^,³^,⁴^,⁵^,⁶^,⁷^,⁸

¹Department of Neurology, Boston Children's Hospital, ²FM Kirby Neurobiology Center, Boston Children's Hospital, ³Department of Neurology, Harvard Medical School, ⁴Medical Genetics Training Program, Harvard Medical School, ⁵Department of Ophthalmology, Boston Children's Hospital, ⁶Department of Ophthalmology, Harvard Medical School, ⁷Broad Institute of M.I.T. and Harvard, ⁸Howard Hughes Medical Institute, ⁹Department of Neurology, Kokura Memorial Hospital, ¹⁰Department of Genetics, Albert Einstein College of Medicine

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Summary

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Amyotrophic Lateral Sclerosis

Islet1 EGF

In Vitro Culture

Embryonic Mice

Motor Neuron Development

Selective Vulnerability

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Isolation and Culture of Oculomotor, Trochlear, and Spinal Motor Neurons from Prenatal Islmn:GFP Transgenic Mice

Isolation and Culture of Oculomotor, Trochlear, and Spinal Motor Neurons from Prenatal Isl^mn:GFP Transgenic Mice