January 7th, 2019
•The goal of this technique is to prepare a highly enriched culture of primary motoneurons (MNs) from murine spinal cord. To evaluate the consequences of mutations causing MN diseases, we describe here the isolation of these isolated MNs and their transfection by magnetofection.
Tags
Related Videos
Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation
Detection of Neuritic Plaques in Alzheimer's Disease Mouse Model
Lectin-based Isolation and Culture of Mouse Embryonic Motoneurons
Detection of Microregional Hypoxia in Mouse Cerebral Cortex by Two-photon Imaging of Endogenous NADH Fluorescence
In Vitro Modeling of Cancerous Neural Invasion: The Dorsal Root Ganglion Model
Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons
Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation
Rapid and Specific Immunomagnetic Isolation of Mouse Primary Oligodendrocytes
Neuronal Differentiation from Mouse Embryonic Stem Cells In vitro
Modeling Stroke in Mice: Focal Cortical Lesions by Photothrombosis
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved