Heparin-Loaded Monovette Preparation and Blood Sampling
1:44
Flow Loop Preparation
2:24
Hemocompatibility Testing
2:51
Whole Blood Count Analysis
3:25
Citrate Plasma Collection
4:05
EDTA and Citrate, Theophylline, Adenosine, and Dipyridamole (CTAD) Plasma Collection
5:19
Thrombin-Anti-Thrombin III (TAT) Quantification
6:48
Scanning Electron Microscopy (SEM) Sample Preparation
7:30
Results: Representative Hemocompatibility Testing
9:02
Conclusion
Transcript
This protocol presents a model for the hemocompatibility investigation of blood-contacting devices according to International Standard Organization guidelines. The model is ideal for mimicking the physiological conditions that correspond to the im
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This protocol describes a comprehensive hemocompatibility evaluation of blood-contacting devices using laser-cut neurovascular implants. A flow loop model with fresh, heparinized human blood is applied to mimic blood flow. After perfusion, various hematologic markers are analyzed and compared to the values gained directly after blood collection for hemocompatibility evaluation of the tested devices.