Isolation, Culture, and Adipogenic Induction of Neural Crest Original Adipose-Derived Stem Cells from Periaortic Adipose Tissue
6.0K Views
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08:31 min
•
March 2nd, 2020
DOI :
March 2nd, 2020
•Yiding Qi*1, Xiang Miao*2, Lian Xu3, Mengxia Fu3, Shi Peng4, Kailei Shi5, Jun Li4, Maoqing Ye5, Ruogu Li1
1Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, 2Shanghai Institute of Nutrition and Health, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 3Shanghai Key Laboratory of Hypertension, Shanghai Institute of Hypertension, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 4Department of Cardiology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, 5Department of Cardiology, Shanghai Key Laboratory of Clinical Geriatric Medicine, Huadong Hospital Affiliated to Fudan University
Chapters in this video
0:04
Introduction
1:24
Periaortic Adipose Tissue (PAAT) Dissection
2:55
Stromal Vascular Cell Fraction (SVCF) Isolation
4:51
Neural Crest Original Adipose-Derived Stem Cell (NCADSC) Culture
6:13
NCADSC Adipogenic Induction
6:42
Results: Representative Adipocyte Differentiation and Characterization
7:58
Conclusion
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