Dissection of Epididymal Fat Pad and Tissue Dissociation
2:38
Antibody Labeling and Fluorescence Activated Cell Sorting (FACS)
4:48
Results: Isolation of Viable Sca1+ Adipose Progenitor Single Cells
5:34
Conclusion
Transcript
This protocol makes it possible to isolate high-quality adipose progenitor cells from mouse epididymal adipose tissue for sensitive downstream analysis. This technique yields highly viable adipose progenitor cells and has been validated in a recen
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We present a simple method to isolate highly viable adipose progenitor cells from mouse epididymal fat pads using fluorescence activated cell sorting.