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Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids

DOI :

10.3791/60704-v

7:19 min

February 18th, 2020

February 18th, 2020

15,457 Views

1Department of Visceral-, Thoracic and Vascular Surgery, University Hospital Carl Gustav Carus, Technische Universität Dresden

This protocol describes an efficient electroporation method for the transfection of four different gastrointestinal organoid entities with larger plasmids (to the extent of 10 kB). It can be performed within one day and does not need extensive preparation or special, cost-intensive electroporation buffers.

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Electroporation Protocol

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