JoVE Logo
Faculty Resource Center

Sign In

A Video Protocol of Retroviral Infection in Primary Intestinal Organoid Culture

DOI :

10.3791/51765-v

9:18 min

August 11th, 2014

August 11th, 2014

29,035 Views

1Department of Genetics, University of Cambridge, 2Wellcome Trust - Medical Research Council Stem Cell Institute, University of Cambridge

This protocol explains primary Lgr5-positve organoid culture and the subsequent performance of retroviral transduction. This enables Cre-inducible overexpression or knockdown of the delivered transgene and allows functional studies to be carried out in the novel in vitro organotypic model system.

Tags

Keywords Organoid Culture

-- Views

Related Videos

article

Protocol for Dengue Infections in Mosquitoes (A. aegypti) and Infection Phenotype Determination

article

Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.

article

Isolation and Primary Culture of Rat Hepatic Cells

article

Real Time Analysis of Metabolic Profile in Ex Vivo Mouse Intestinal Crypt Organoid Cultures

article

A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids

article

Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin

article

Apoptosis Induction and Detection in a Primary Culture of Sea Cucumber Intestinal Cells

article

Organoid-Derived Epithelial Monolayer: A Clinically Relevant In Vitro Model for Intestinal Barrier Function

article

Generation of Murine Primary Colon Epithelial Monolayers from Intestinal Crypts

article

Culture Methods to Study Apical-Specific Interactions using Intestinal Organoid Models

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved