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Time-lapse Imaging of Bacterial Swarms and the Collective Stress Response

DOI :

10.3791/60915-v

May 23rd, 2020

May 23rd, 2020

7,613 Views

1Department of Molecular Biology & Biochemistry, University of California, Irvine, 2Department of Physics & Astronomy, University of California, Irvine, 3Department of Veterinary and Animal Sciences, University of Copenhagen

We detail a simple method to produce high-resolution time-lapse movies of Pseudomonas aeruginosa swarms that respond to bacteriophage (phage) and antibiotic stress using a flatbed document scanner. This procedure is a fast and simple method for monitoring swarming dynamics and may be adapted to study the motility and growth of other bacterial species.

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Time lapse Imaging

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