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High-Throughput Live Imaging of Microcolonies to Measure Heterogeneity in Growth and Gene Expression

DOI :

10.3791/62038-v

April 18th, 2021

April 18th, 2021

4,394 Views

1Center for Genomics and Systems Biology, Department of Biology, New York University

Yeast growth phenotypes are precisely measured through highly parallel time-lapse imaging of immobilized cells growing into microcolonies. Simultaneously, stress tolerance, protein expression, and protein localization can be monitored, generating integrated datasets to study how environmental and genetic differences, as well as gene-expression heterogeneity among isogenic cells, modulate growth.

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