Sign In

JoVE Journal

Cancer Research

A subscription to JoVE is required to view this content.

Drug Screening of Primary Patient Derived Tumor Xenografts in Zebrafish

7.8K Views

09:50 min

April 10th, 2020

April 10th, 2020

7,824 Views

0:04

Introduction

0:54

Fluorescently Labeling Cells with DiI(1,1 -Dioctadecyl-3,3,3 ,3 -Tetramethylindocarbocyanine Perchlorate)

2:10

Microinjecting Zebrafish Larvae

4:37

Setting up Drug Screen with Xenografted Zebrafish

6:22

Imaging Xenografted Zebrafish and Quantifying Fluorescence

8:36

Results: Xenografted Tumor Area

9:22

Conclusion

Transcript

Our zebrafish xenograft drug screening workflow allows for automated imaging and quantification of human cancer cells and their response to drug treatment. It can be used to rapidly test the response of a patience's cancer cells to known drugs or

Sign in or start your free trial to access this content

Zebrafish xenograft models allow for high-throughput drug screening and fluorescent imaging of human cancer cells in an in vivo microenvironment. We developed a workflow for large scale, automated drug screening on patient-derived leukemia samples in zebrafish using an automated fluorescence microscope equipped imaging unit.

We use cookies to enhance your experience on our website.

By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.

Learn More