48 Hours Post Fertilization (HPF) Zebrafish Embryo Dechorionation
1:42
Dithiothreitol (DTT) Treatment
2:17
Embryo Dissociation
3:13
Flow Cytometric Sample Preparation
3:51
Flow Cytometry
6:10
Results: Representative Effects of Morpholino (MO)-Mediated Transcript Reduction on Red Blood Cell Production Numbers During Embryogenesis
7:01
Conclusion
Transcript
This protocol is significant because it allows the knockdown and over expression of genes in a developing fish, and the quantitation of those downstream effects on blood cells. This protocol is quick, easy to perform, economical, and easy to autom
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This assay is a simple method to quantitate hematopoietic cells in developing embryonic zebrafish. Blood cells from dissociated zebrafish are subjected to flow cytometry analysis. This allows the detection of blood defects in mutant animals and after genetic modification.