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High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology

7.6K Views

08:19 min

May 28th, 2020

DOI :

10.3791/61132-v

May 28th, 2020


Transcript

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RT qPCR

Chapters in this video

0:05

Introduction

0:46

Worm-to-CT Worm Lysis

2:08

Reverse Transcription (RT) for Bulk Samples

2:38

Target Specific Preamplification

3:19

Exonuclease I Treatment

3:53

Sample Mix Preparation

4:48

Nanofluidic Chip Priming and Loading

6:00

Results: Representative Worm-to-CT Analysis

7:45

Conclusion

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