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Automating Aggregate Quantification in Caenorhabditis elegans

DOI :

10.3791/62997-v

7:50 min

October 14th, 2021

October 14th, 2021

2,177 Views

1Department of Microbiology and Cell Science, University of Florida, 2Department of Computer and Information Science and Engineering, University of Florida

The following protocol describes the development and optimization of a high-throughput workflow for worm culturing, fluorescence imaging, and automated image processing to quantify polyglutamine aggregates as an assessment of changes in proteostasis.

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Automating Aggregate Quantification

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