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JoVE Journal

Neuroscience

This content is Open Access.

Visualizing Synaptic Degeneration in Adult Drosophila in Association with Neurodegeneration

5.9K Views

06:10 min

May 13th, 2020

May 13th, 2020

5,877 Views

0:04

Introduction

0:41

Thorax Isolation and Fixation

2:12

Flash Freezing and Thorax Bisection

3:41

Structural Staining

4:08

Tissue Mounting

4:57

Results: Representative Synaptic Integrity Assessment

5:46

Conclusion

Transcript

The goal of this procedure is to dissect the dorsal longitudinal muscle (DLM) tissue to assess the structural integrity of DLM neuromuscular junctions (NMJs) in neurodegenerative disease models using Drosophila melanogaster.

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