Measurement of Insulin- and Contraction-Stimulated Glucose Uptake in Isolated and Incubated Mature Skeletal Muscle from Mice

5.2K Views

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08:01 min

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May 16th, 2021

DOI :

10.3791/61398-v

May 16th, 2021

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Rasmus Kjøbsted¹, Kohei Kido¹, Jeppe K. Larsen¹, Nicolas O. Jørgensen¹, Jesper B. Birk¹, Ylva Hellsten², Jørgen F. P. Wojtaszewski¹

¹Section of Molecular Physiology, Department of Nutrition, Exercise and Sports, University of Copenhagen, ²Section of Integrative Physiology, Department of Nutrition, Exercise and Sports, University of Copenhagen

Summary

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Physiological Interventions

Pharmacological Compounds

Tibialis Anterior Muscle

EDL Muscle

Muscle Dissection

Data Collection Calibration

Oxygenation

Chapters in this video

0:04

Introduction

0:39

Mouse Soleus and Extensor Digitorum Longus (EDL) Harvest

3:15

Insulin-Stimulated Glucose Uptake

4:18

Contraction-Stimulated Glucose Uptake

4:57

Skeletal Muscle Homogenization and Radiolabeled 2-Deoxyglucose and Mannitol Determination

5:58

Results: Representative Mouse Soleus and EDL Muscle Metabolic Activity Analysis

7:22

Conclusion

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