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Measurement of Insulin- and Contraction-Stimulated Glucose Uptake in Isolated and Incubated Mature Skeletal Muscle from Mice

5.6K Views

08:01 min

May 16th, 2021

DOI :

10.3791/61398-v

May 16th, 2021


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Insulin Stimulation

Chapters in this video

0:04

Introduction

0:39

Mouse Soleus and Extensor Digitorum Longus (EDL) Harvest

3:15

Insulin-Stimulated Glucose Uptake

4:18

Contraction-Stimulated Glucose Uptake

4:57

Skeletal Muscle Homogenization and Radiolabeled 2-Deoxyglucose and Mannitol Determination

5:58

Results: Representative Mouse Soleus and EDL Muscle Metabolic Activity Analysis

7:22

Conclusion

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