Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin
6.2K Views
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11:17 min
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March 10th, 2021
DOI :
March 10th, 2021
•Di Zhang*1,2, Kefang Liu*3,4, Dan Lu*2,5, Pengyan Wang1,2, Qingxu Zhang1,2, Peipei Liu2, Yingze Zhao2, Yan Chai4, Jianxin Lyu1, Jianxun Qi4, William J. Liu1,2
1School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, 2NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 3Faculty of Health Sciences, University of Macau, 4CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, 5Savaid Medical School, University of Chinese Academy of Sciences
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Chapters in this video
0:04
Introduction
0:54
E. coli Transformation
1:46
Culture Inoculation
2:59
Bacteria Harvest
3:28
Inclusion Body Purification
4:27
MHC Complex Refolding
4:53
MHC H Chain and Beta2m Injection and Dilution
5:42
Refolded Protein Concentration Determination
6:51
Crystallization, Data Collection, and Processing
7:41
Structure Determination and Analyses
8:33
Results: Representative Bat MHC I Beta2-Microglobulin Stability and Structure Analysis
10:21
Conclusion
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