Unraveling the Role of Discrete Areas of the Rat Brain in the Regulation of Ovulation through Reversible Inactivation by Tetrodotoxin Microinjections
3.0K Views
•
11:04 min
•
September 3rd, 2020
DOI :
September 3rd, 2020
•Transcript
Chapters in this video
0:04
Introduction
0:47
Construction of Bilateral Cannulas, Obturators, and Caps
2:49
Construction of Microinjectors
4:10
Animal Maintenance and Vaginal Smears
5:31
Microinjection of TTX or Vehicle Solutions into Freely Moving Rats
7:27
Tissue Processing
8:44
Results: Percentage of Ovulating Animals and Median of the Number of Ova Shed after Microinjection with Artificial Cerebrospinal Fluid (ACSF) or Tetrodotoxin (TTX)
10:08
Conclusion
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