Isolation of Proximal Fluids to Investigate the Tumor Microenvironment of Pancreatic Adenocarcinoma

3.2K Views

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05:44 min

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November 5th, 2020

DOI :

10.3791/61687-v

November 5th, 2020

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Greta Donisi*¹, Marialuisa Barbagallo*², Giovanni Capretti¹^,³, Gennaro Nappo¹^,³, Panteleimon G. Takis⁴^,⁵^,⁶, Alessandro Zerbi¹^,³, Federica Marchesi²^,⁷, Nina Cortese²

¹Section of Pancreatic Surgery, Humanitas Clinical and Research Center-IRCCS, ²Department of Immunology and Inflammation, Humanitas Clinical and Research Center-IRCCS, ³Department of Biomedical Sciences, Humanitas University, ⁴Giotto Biotech S.R.L., ⁵Section of Bioanalytical Chemistry, Division of Systems Medicine, Department of Metabolism, Digestion and Reproduction, Imperial College London, ⁶National Phenome Centre, Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Imperial College London, ⁷Department of Medical Biotechnology and Translational Medicine, University of Milan

Summary

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Pancreatic Adenocarcinoma

Tumor Microenvironment

Pancreatic Juice

Proximal Fluid Isolation

Interstitial Fluid TIF

Centrifugation Protocol

Cold Storage

Sample Analysis

Chapters in this video

0:04

Introduction

1:03

Isolation and Processing of Pancreatic Juice

2:20

Isolation of Tumor Interstitial Fluid (TIF) from Subcutaneous Tumors

4:03

Results: Metabolomic Content in Pancreatic Juice

5:14

Conclusion

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