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Isolation and Characterization of Patient-derived Pancreatic Ductal Adenocarcinoma Organoid Models

DOI :

10.3791/60364-v

January 14th, 2020

January 14th, 2020

10,864 Views

1Moores Cancer Center, University of California San Diego, 2Department of Surgery, Division of Surgical Oncology, University of California San Diego

Patient-derived organoid cultures of pancreatic ductal adenocarcinoma are a rapidly established 3-dimensional model that represent epithelial tumor cell compartments with high fidelity, enabling translational research into this lethal malignancy. Here, we provide detailed methods to establish and propagate organoids as well as to perform relevant biological assays using these models.

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Isolation

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