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Applications of Immobilization of Drosophila Tissues with Fibrin Clots for Live Imaging

DOI :

10.3791/61954-v

8:52 min

December 22nd, 2020

December 22nd, 2020

2,609 Views

1Cell & Developmental Biology, School of Life Sciences, University of Dundee

This protocol describes applications of sample immobilization using Fibrin clots, limiting drifting, and allowing the addition and washout of reagents during live imaging. Samples are transferred to a drop of Fibrinogen containing culture medium on the surface of a coverslip, after which polymerization is induced by adding thrombin.

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Immobilization

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