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Refined CLARITY-Based Tissue Clearing for Three-Dimensional Fibroblast Organization in Healthy and Injured Mouse Hearts

DOI :

10.3791/62023-v

May 16th, 2021

May 16th, 2021

4,309 Views

1Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine, 2Division of Molecular Cardiovascular Biology, Cincinnati Children's Hospital Medical Center, 3Confocal Imaging Core, Cincinnati Children's Hospital Medical Center

A refined method of tissue clearing was developed and applied to the adult mouse heart. This method was designed to clear dense, autofluorescent cardiac tissue, while maintaining labeled fibroblast fluorescence attributed to a genetic reporter strategy.

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