Synthetic Cystic Fibrosis Medium (SCFM2) Preparation
1:03
Bacterial Cell Suspension Preparation
2:36
Pseudomonas aeruginosa Aggregate Visualization and Isolation
4:48
Image Analysis
6:15
Results: Analysis of Pseudomonas aeruginosa Aggregates
7:10
Conclusion
Transcript
Our protocol provides a way for us to study antibiotic-resistant bacteria in a nutritional and physical environment similar to how they likely exist in vivo. One of the greatest advantages of this technique is the ability to capture high-resolutio
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Synthetic cystic fibrosis sputum medium (SCFM2) can be utilized in combination with both confocal laser scanning microscopy and fluorescence-activated cell sorting to observe bacterial aggregates at high resolution. This paper details methods to assess aggregate populations during antimicrobial treatment as a platform for future studies.