Incisor Dissection and Isolation of the Proximal End Tissue
3:45
Tissue Fixation
4:19
Results: Analysis of the Isolated Cervical Loops
5:51
Conclusion
Transcript
The Trowell-type organ culture is used to culture tissue explants. Here, we show how to use this method to study the stem cells in continuously-growing teeth. With this technique, we can study the stem cells and their behavior in the niche and scr
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The Trowell-type organ culture method has been used to unravel complex signaling networks that govern tooth development and, more recently, for studying regulation involved in stem cells of the continuously growing mouse incisor. Fluorescent-reporter animal models and live-imaging methods facilitate in-depth analyses of dental stem cells and their specific niche microenvironment.