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Practical Aspects of Sample Preparation and Setup of 1H R Relaxation Dispersion Experiments of RNA

DOI :

10.3791/62470-v

8:17 min

July 9th, 2021

July 9th, 2021

4,146 Views

1Department of Medical Biochemistry and Biophysics, Karolinska Institutet

We present a protocol to measure micro- to millisecond dynamics on 13C/15N-labeled and unlabeled RNA with 1H R relaxation dispersion nuclear magnetic resonance (NMR) spectroscopy. The focus of this protocol lies in high-purity sample preparation and setup of NMR experiments.

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RNA Sample Preparation

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