JoVE Logo
Faculty Resource Center

Sign In

Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies

DOI :

10.3791/62971-v

November 4th, 2021

November 4th, 2021

2,033 Views

1Department of Biological and Environmental Sciences, Bioscience Institute, Sao Paulo State University (UNESP), 2Biodiversity of Coastal Environments Postgraduate Program, Bioscience Institute, Sao Paulo State University (UNESP)

This article describes a sample preparation method based on heat-inactivation to preserve endogenous peptides avoiding degradation post-mortem, followed by relative quantitation using isotopic labeling plus LC-MS.

Tags

Sample Preparation

-- Views

Related Videos

article

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

article

An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering

article

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation

article

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

article

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

article

Plant Sample Preparation for Nucleoside/Nucleotide Content Measurement with An HPLC-MS/MS

article

A Sample Preparation Pipeline for Microcrystals at the VMXm Beamline

article

Large-Scale Multi-Omics Genome-Wide Association Studies (Mo-GWAS): Guidelines for Sample Preparation and Normalization

article

Preparation of High-Temperature Sample Grids for Cryo-EM

article

Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved