Results: Determining Labelled Peptides Using Mass Spectroscopy
7:19
Conclusion
Transcript
Here, we describe an instructional method based on a fast heat inactivation of protease to preserve natural intracellular peptides, avoid degradation in cells and tissues, followed by relative quantitation of peptides using isotopic labeling. This
Sign in or start your free trial to access this content
This article describes a sample preparation method based on heat-inactivation to preserve endogenous peptides avoiding degradation post-mortem, followed by relative quantitation using isotopic labeling plus LC-MS.