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Leukodepletion Filters-Derived CD34+ Cells As a Cell Source to Study Megakaryocyte Differentiation and Platelet Formation

DOI :

10.3791/62499-v

May 20th, 2021

May 20th, 2021

3,319 Views

1BPPS UMR-S 1255, FMTS, Université de Strasbourg, INSERM, EFS Grand Est

This protocol describes in detail all the steps involved in obtaining leukofilter-derived CD34+ hematopoietic progenitors and their in vitro differentiation and maturation into proplatelet-bearing megakaryocytes that are able to release platelets in the culture medium. This procedure is useful for in-depth analysis of cellular and molecular mechanisms controlling megakaryopoiesis.

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