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Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles

DOI :

10.3791/62504-v

8:02 min

May 14th, 2021

May 14th, 2021

4,279 Views

1Istituti Clinici Scientifici Maugeri IRCCS, 2Dipartimento di Scienze Biomediche e Cliniche “L. Sacco”, Università di Milano, 3Dipartimento di Biotecnologie e Bioscienze, Università di Milano-Bicocca

This paper aims to provide a protocol for the isolation and culture of primary cancer-associated fibroblasts from a syngeneic murine model of triple-negative breast cancer and their application for the preclinical study of novel nanoparticles designed to target the tumor microenvironment. 

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