April 5th, 2013
•Cellular viability depends on timely and efficient management of protein misfolding. Here we describe a method for visualizing the different potential fates of a misfolded protein: refolding, degradation, or sequestration in inclusions. We demonstrate the use of a folding sensor, Ubc9ts, for monitoring proteostasis and aggregation quality control in live cells using 4D microscopy.
Related Videos
Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells in Xenopus laevis
Imaging Protein-protein Interactions in vivo
Live Imaging of Apoptotic Cell Clearance during Drosophila Embryogenesis
Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer
In Vitro Aggregation Assays Using Hyperphosphorylated Tau Protein
Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions
Methods to Study Changes in Inherent Protein Aggregation with Age in Caenorhabditis elegans
4D Microscopy of Yeast
FLIM-FRET Measurements of Protein-Protein Interactions in Live Bacteria.
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved