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4D Imaging of Protein Aggregation in Live Cells

DOI :

10.3791/50083-v

8:59 min

April 5th, 2013

April 5th, 2013

16,876 Views

1Department of Cell and Developmental Biology, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem

Cellular viability depends on timely and efficient management of protein misfolding. Here we describe a method for visualizing the different potential fates of a misfolded protein: refolding, degradation, or sequestration in inclusions. We demonstrate the use of a folding sensor, Ubc9ts, for monitoring proteostasis and aggregation quality control in live cells using 4D microscopy.

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4D Imaging

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